foxrabiesblueprint.org

Supplies needed for laboratory-based rabies diagnosis using the fluorescence antibody test

Equipment

  • Laboratory furniture (work bench, cubicles, chairs, shelf)
  • Necropsy instruments (scalpel, bone saw, scissors, knife, tweezers)
  • Steriliser/autoclave
  • Specimen storage containers
  • Post-stain rinse containers
  • Refrigerator
  • Freezer (-20°C)
  • Pipette and tips
  • Fluorescent microscope
  • Non-fluorescing microscopic slides
  • Incubator (37°C) – may not be necessary in tropical countries
  • Book/submission forms for reporting the diagnosis, following national or regional guidelines.

- Personal protective wear: lab coats, gum boots, rubber gloves, disposable gloves, masks

Chemicals

  • Acetone
  • Distilled water or highly purified water
  • Positive and negative brain samples for controls
  • FITC [1]-conjugated anti-rabies antibodies
  • (PBS) phosphate buffered isotonic saline solution at pH 7.4 to 7.6 (0.01 M phosphate buffer, pH 7.4, with 0.138 M NaCl [2] and 0.0027 M KCl [3])
  • Mountant (immersion oil)

Click here for more detailed information on equipment, reagents and protocols for diagnosis of rabies in animals by fluorescent antibody testing.

JPEG - 49.7 kb
Photo courtesy of Serengeti Carnivore Disease Project

[1Fluorescein isothiocyanate

[2Sodium chloride

[3Potassium chloride


PARTNER LINKS

Home | Contact | Site Map |
Version 1 - Last updated November 2012